$ awk ‘FNR==NR{a[$1]=$2 FS $3;next}{ print $0, a[$1]}’ file2 file1
4050 S00001 31228 3286 0 12.1 23.6
4050 S00012 31227 4251 0 12.1 23.6
4049 S00001 28342 3021 1 14.4 47.8
4048 S00001 46578 4210 0 23.2 43.9
4048 S00113 31221 4250 0 23.2 43.9
4047 S00122 31225 4249 0 45.5 21.6
4046 S00344 31322 4000 1
