跪求英译中!不要在线翻译的,谢谢!

跪求英译中!不要在线翻译的,谢谢!Antigene peptide nucleic acid specifically inhibits MYCN expression in human neuroblastoma cells leading to cell growth inhibition and apoptosisRoberto Tonelli,Stefania Purgato,Consuelo Camerin,Raffaele Fronza,Fabrizio Bologna,Simone Alboresi,Monica Franzoni,Roberto Corradini,Stefano Sforza,Andrea Faccini,Jason M. Shohet,Rosangela Marchelli,and Andrea Pession Department of Pediatrics, University of Bologna,Sant’Orsola-Malpighi Hospital, Bologna, Italy; Department of Organic and Industrial Chemistry, University of Parma, Parma,Italy; and 3Center for Cell and Gene Therapy, Texas Children’s Cancer Center, Baylor College of Medicine, Houston, TexasAbstractWe developed an antigene peptide nucleic acid (PNA) forselective inhibition of MYCN transcription in neuroblastomacells, targeted against a unique sequence in theantisense DNA strand of exon 2 of MYCN and linked atits NH2 terminus to a nuclear localization signal peptide.Fluorescence microscopy showed specific nuclear deliveryof the PNA in six human neuroblastoma cell lines: GI-LINand IMR-32 (MYCN-amplified/overexpressed); SJ-NKPand NB-100 (MYCN-unamplified/low-expressed);and GI-CA-N and GI-ME-N (MYCN-unamplified/unexpressed).Antiproliferative effects were observable at24 hours (GI-LI-N, 60%; IMR-32, 70%) and peaked at72 hours (GI-LI-N, 80%; IMR-32, 90%; SK-N-KP, 60%;NB-100, 50%); no reduction was recorded for GI-CA-Nand GI-ME-N (controls). In MYCN-amplified/overexpressedIMR-32 cells and MYCN-unamplified/lowexpressedSJ-N-KP cells, inhibition was recorded ofMYCN mRNA (by real-time PCR) and N-Myc (Westernblotting); these inhibitory effects increased over 3 daysafter single treatment in IMR-32. Antigene PNA inducedG1-phase accumulation (39–53%) in IMR-32 and apoptosis(56% annexin V–positive cells at 24 hours inIMR-32 and 22% annexin V–positive cells at 48 hoursin SJ-N-KP). Selective activity of the PNA was shownby altering three point mutations, and by the observationthat an antigene PNA targeted against the noncodingDNA strand did not exert any effect. These findingscould encourage research into development of anantigene PNA–based tumor-specific agent for neuroblastoma(and other neoplasms) with MYCN expression.[Mol Cancer Ther 2005;4(5):779–86]