求助蛋白表达中的his标签的作用

his标签蛋白就是：六个组氨酸。一般存在于重组蛋白氮端或碳端,便于纯化该重组蛋白,组氨酸可以特异性的结合镍离子,带有组氨酸标签的重组蛋白可以被镍柱吸附,从而达到纯化的目的。也就是在表达载体上面,特别加六个组氨酸的碱基序列,目的就是纯化。一下是英文的解释,你看一眼A recombinant protein can be tagged N-terminally or C-ternimally with a 6XHis tag。This tag is better in prokaryotic system rather than mammalian system。6X His tag is an affinity tag which allows the tagged recombinant protein to be purified in the process of affinity purification。6XHis tag binds to NTA-agarose。The resin contains nickle ions which binds to Histidine tag。