英语翻译One milliliter spore suspension of the concentration 2×1

英语翻译One milliliter spore suspension of the concentration 2×107 spores/ml was used as a inoculum in each experiment.Each flask,containing medium was inoculated with 1 ml of spore suspension followed by mixing and incubation at 30℃,inside the humidity-controlled incubator.Methanol was added to the medium before inoculation.One flask was harvested every day for the estimation of citric acid produced and sugar consumed,till wholesugar consumed.Sampling was continued until all the sugar of the medium was consumed.Sugar was estimated by the phenol sulphuric acid method of Dubois et al.and citric acid by the acetic anhydride and pyridine method of Marier and Boulet.The present work consisted of comparison and selection of suitable carrier (bagasse/wheat bran) for citric acid production by SSF.The effects of differentparameters including moisture content,particle size,sugar level and methanol concentration in the medium,have been studied.